SODIUM CITRATE

Trisodium citrate is used as an anticoagulant for coagulation investigations. It works as an anticoagulant as it forms complexes with metal ions such as calcium inhibiting the coagulation cascade. Anticoagulation with trisodium citrate is reversible.

DBO Phlecocuum® Citrate tubes contain buffered citrate in accordance with recommendations:

• 0.105 M or 0.109 M of buffered trisodium citrate solution, equivalent to 3.2% trisodium citrate The blood to additive ratio is 9:1.

The citrate solution in the acts as DBO Phlecocuum® a buffer. Therefore, no additional buffer substances are added as these can adversely affect laboratory analysis.

DBO Phlecocuum® Citrate tubes are also suitable for carrying out special test procedures such as the platelet function test PFA-100®*. Special tubes and the associated additional costs are therefore unnecessary.

FILL LINE MARKING

The significance of the correct ratio of blood to additive for coagulation samples is well documented. The correct fill amount is critical for correct coagulation analysis. All DBO Phlecocuum® coagulation tubes have a mark indicating the minimum fill level.

CENTRIFUGING CONDITIONS

For coagulation analysis different plasma specifications can be obtained from the citrated blood.

• Platelet rich plasma:150-200g for 5 minutes at 18-25°C
• Platelet poor plasma:2000-2500g for 10-15 minutes at 18-25°C
• Platelet free plasma: >3000g for 15-30 minutes at 18-25°C

MIXING RECOMMENDATION

Citrate tubes should be gently inverted 180° and back 3-4 times.

SPECIFICATION

Specimen: Plasma or Whole

Additive: Sodium citrate 3.2% or 3.8% (9NC)

Cap color: Blue

Tube Size: 13x75mm

Draw Volume: 1.8mL, 2.7mL

Max. draw volume Tolerance: ±10%